1148 Skewing of M1/M2 macrophage balance is related to photoaging via reduction of the antioxidative enzyme PRDX4

Ultraviolet irradiation directly affects the skin and promotes photoaging by causing depletion of antioxidative enzymes. We recently reported that M1/M2 macrophage ratio is altered in photo-aged skin. However, relationship between balance expression enzymes has not been fully investigated. Here, we show skewing related to reduction enzyme peroxiredoxin 4 (PRDX4) it may lead photoaging. performed a bulk RNA-seq analysis mRNA, with focus on oxidation-related dermal fibroblasts cultured supernatant M1 macrophages (M1) or M2 (M2). found induced multiple oxidative enzymes, while fibroblasts. Among significantly genes focused PRDX4 as properties, such hydrogen peroxide detoxification, its level fluctuates M2. observed skin; was positively correlated number cells negatively ratio. To investigate effects photoaging, examined modification collagen. high acrolein accumulation collagen model after application peroxide, suggesting carbonyl had occurred. Furthermore, COL I both skin, only but also expression. Our results demonstrate play an important role inhibiting collagen, which are major known features Taken together, maintenance present possible new target for